Protein_Domain

Part:BBa_K1110003:Experience

Designed by: Lydia Studdard   Group: iGEM13_Georgia_State   (2013-09-18)


Applications of BBa_K1110003

Mambalgin-1 was expressed in Pichia pastoris and confirmed with colony PCR. Expression was shown with a western blot and coomassie stain. The coomassie stain resulted in a band near 9kDa, which was the expected band size. Additionally, further purification of the supernatant was not needed after initial centrifugation. Thus, protein expression of Mambalgin-1 in P. pastoris required a minimal amount of steps.

T--Georgia State--Pichia Mambalgin1 Coomassie.png


T--Georgia State--Mambalgin Ppastoris Coomassie.png

Figure 1: Coomassie Gel Stain of Mambalgin-1 in Pichia pastoris Protein Expression. Coomassie stain of SDS-PAGE run at 100 volts for 30 minutes.

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